Product Name:
Rat Plasminogen Activator Inhibitor 1 (PAI-1) ELISA Kit
Cat.#:
JL11181
Regulatory Status:
Alternative:
SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.31-20ng/mL
Sensitivity:
0.12ng/mL
Specificity:
This kit recognizes Rat PAI-1 in samples. No significant cross-reactivity or interference between Rat PAI-1 and analogues was observed.
Sample Type:
Plasma
Assay Procedure:

Research Area:
Metabolism
Product Datasheets
| PAI-1
Plasminogen activator inhibitor-1 (PAI-1) also known as endothelial plasminogen activator inhibitor (serpin E1) is a protein that is encoded by the SERPINE1 gene. Elevated PAI-1 is a risk factor for thrombosis and atherosclerosis.PAI-1 is a serine protease inhibitor (serpin) that functions as the principal inhibitor of tissue-type plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1).| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 20 | 10 | 5 | 2.5 | 1.25 | 0.62 | 0.31 | 0 |
OD | 2 | 1.64 | 1.22 | 0.84 | 0.5 | 0.34 | 0.25 | 0.09 |
Corrected OD | 1.91 | 1.55 | 1.13 | 0.75 | 0.41 | 0.25 | 0.16 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Rat PAI-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Plasma(n=8) | 92-105 | 102 |
| Linearity
Samples were spiked with high concentrations of Rat PAI-1 and diluted with Universal Diluent to produce samples with values within the range of the assay.Plasma(n=4) | ||
1 : 2 | Range (%) | 88-96 |
Average (%) | 92 | |
1 : 4 | Range (%) | 87-108 |
Average (%) | 98 |






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